CURRICULUM VITAE
Prof. ALIK HONIGMAN
Birth date: 1 January 1942
Marital status: Married, three children
1959-1962: Military service, Israel Defense Forces (officer)
 
Education
1967, B.Sc., The Hebrew University of Jerusalem, Israel. Faculty of Science. Main Subject: Microbiology and Genetics.
1969, M.Sc., The Hebrew University-Hadassah Medical School, Jerusalem, Israel. Dept. Chemical Microbiology.
Thesis: "Genetic control of the life cycle of Bacteriophage P1"
 
1970-1975, Ph.D. student, Assistant and Instructor at the, Dept. of Molecular Genetics. The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Thesis: "Genetics and Biochemical Studies of the early Regulation of Bacteriophage lambda".
 
Previous employment
1995-present Dept. of Virology
1966-present Chairman of the scientific advisory board of Promega BioTechnology company.
1994-1995 Promega Corp. and University of Wisconsin, Visiting Professor of Molecular Virology
1992- 1997 Institute of Microbiology - Head of Curriculum Committee
1990-1995 Dept. of Molecular Genetics, Hebrew University-Hadassah Medical School - Head of Department
1992-1995 Dept. of Molecular Genetics, Hebrew University- Hadassah Medical School, Associate Professor
1983-1992 Dept. of Molecular Genetics, Hebrew University- Hadassah Medical School, Senior Lecturer
1984-1985 NIH, NCI-FCRF, Fredrick, Maryland, Visiting Scientist
1977-1982 Dept. of Molecular Genetics, Hebrew University- Hadassah Medical School, Lecturer
1975-1977 McArdle Laboratory for Cancer Research, University Of Wisconsin, Madison, WI - Research Associate in Prof. Szybalski's laboratory
 
Publications
Prof. Alik Honigman
 
1. Olitzki, A., D. Godinger, M. Israeli and A. Honigman (1967). Studies on Atypica mycobacteria. Glycols, mono- and polyhydric alcohols as growth Promoting and inhibiting substances. Estratto dal Boll 1st sieroterMilanese 46:5-6
2. Olitzki, A., H. Haas, D. Godinger, M. Israeli and A. Honigman (1967).Studies on atypical Mycobacteria in Israel. Path. Microbiol. 30:433-488.
3. Olitzki, A., D. Godinger, M. Israeli and A. Honigman (1967). In vitro Effects of some chemotherapeutic agents on mycobacteria. Applied Microbiology 15:994-1001.
4. Echols, H., L. Green, A.B. Oppenheim and A. Honigman (1973). Role of The cro gene in bacteriophage lambda development. J. Mol. Biol. 80:203-216.
5. Oppenheim, A., A. Honigman and A.B. Oppenheim (1974). Interference with phage lambda cro gene function by a colicin-tolerant E. coli mutant. Virology 61:1-10.
6. Oppenheim, A.B., A. Honigman, A. Oppenheim and W. Stevens (1975).Interaction between bacteriophage lambda and its bacterial host: regulation Of developmental pathways, in: Dynamic Aspects of Host Parasite Relationships, A. Zuckerman, Editor. A. Halsted Press: New York, Toronto, Israel Universities Press, Jerusalem, pp. 40-55.
7. Honigman, A., A. Oppenheim and W. Stevens (1975). A pleiotropic Regulatory mutation in lambda bacteriophage. Mol. Gen. Genet. 138:85-111.
8. Honigman, A., S.L. Hu, R. Chase, and W. Szybalski (1976). 4S oop RNA is a leader sequence for the immunity-establishment transcription in coliphage lambda. Nature 262:112-116.
9. Rosenvold, E. and A. Honigman (1977). Mapping of Ava 1 and Xma 1 cleavage sites in bacteriophage lambda DNA including a new technique of DNA digestion in agarose gels. Gene. 2:273-288.
10. Fiandt, M., W. Szybalski, A. Honigman and E. Rosenvold (1977). Precise measurement of the b2 deletion in E. coli phage lambda. Gene. 3:289-293.
11. Szybalski, E., M. Fiandt, A. Honigman, E. Rosenvold and W. Szybalski (1977). A deletion of the p-Q (nin) region of phage lambda b2mm21 conferring partial N21 independence. Gene. 2:294-296.
12. Fuchs, C., E. Rosenvold, A. Honigman and W. Szybalski (1979). A simple method for identifying the palidromic sequences recognized by restriction endonucleases: the nucleotide sequence of the Ava 11 site. Gene. 4:1-23.
13. Honigman, A., S.L. Hu and W. Szybalski (1979). Regulation of integration by coliphage lambda. Virology 92:542-556.
14. Enquist, L., A. Honigman, S.L. Hu and W. Szybalski (1979). Expression of int gene function in ColE1 hybrid plasmids carrying the C fragment of bacteriophage lambda. Virology 92:557-560.
15. Fuchs, C., E. Rosenvold, A. Honigman and W. Szybalski (1980).Identification of palidromic sequences recognized by restrictases, as based on the tabularized sequencing data for seven viral and plasmid DNAs. Gene. 10:357-370.
16. Honigman, A., A. Oppenheim, B. Hohn and T. Hohn (1981). Plasmid vectors for positive selection of hybrid plasmid: Specified cloning vehicle for isolation of transcriptional determination signals. Gene. 13:289-298.
17. Honigman, A. Cloning and characterization of an N-resistant transcriptional terminator in bacteriophage lambda. Gene. 13:299-309.
18. Hyman, H. and A. Honigman (1982). The use of the plasmid pHA10 in the isolation of lambda PL promoter mutations. Mol. Gen. Genet. 185:515-517.
19. Becker, Y., Y. Shtram, D. Snipper, Y. Asher, E. Tavor, Y. Gordon, D.Gilden, M. Vallish, J. Hadar, O. Becker, A. Cohen, A. Laban and A. Honigman(1982). Herpes Simplex virus type 1 Thymidine kinase gene controls expression in eukaryotic and prokaryotic cells and viral pathogenesis in mice In: International Symposium of Herpes Virus: Clinical, Pharmalogical and Basic Aspects. Tokushima City, Japan. Ed. W. Prusoff, Y.C. Cheng, H.Shiota, Experta Medica, Amsterdam, The Netherlands, pp. 57-73.
20. Honigman, A. and A. Panet (1983). A unique sequence in murine leukemia virus long terminal repeat functions as a termination signal for transcription in Escherichia coli. J. Virol. 45:456-461.
21. Becker, Y., Y. Shtram, A. Honigman, A. Laban and A. Cohen (1983)Expression of herpes simplex tk gene in E. coli. Gene. 21:51-58.
22. Honigman, A., C. Kronman, I. Nur, N. Greenberg and S. Rottem (1984).Cloning of L-2 DNA in Escherichia coli pOL4 plasmid. Isr. J. Med. Sci. 20:793-796.
23. Broza, M., B. Sneh, A. Yawetz, U. Oron and A. Honigman (1984).Commercial application of Bacillus thuringiensis var. entomocidus to cotton fields for the control of Spodoptera littoralis Boisduval (Lepidoptera,Noctuidae). J. Econ. Entom. 77:1530-1533.
24. Oron, U., M. Skolover, A. Yawetz, M. Broza, B. Sneh and A. Honigman (1985). Ultrastructural changes in larval midgut epithelium of Spodoptera littoralis following ingestion of endotoxin of Bacillus thuringiensis var.entomocidus. J. Invertebr. Pathol. 45:353-355.
25. Honigman, A., A. Bar-Shira, H. Silberberg and A. Panet (1985). Generation of a uniform 3' and RNA of murine leukemia virus. J. Virol.53:330-334.
26. Honigman, A., J. Mahajna, S. Altuvia, S. Koby, D. Teff, H.Locker-Giladi, H. Hyman, C. Kronman and A. Oppenheim (1985). Plasmid vectors designed for the analysis of transcription termination signals. Gene. 36:131-141.
27. Oppenheim, A.B., J. Mahajna, S. Altuvia, S. Koby, D. Teff, H.Locker-Giladi, H. Hyman, C. Kronman and A. Honigman (1985). Utilization of control elements for gene expression studies in E. coli, in: Recombinant DNA Research and Viruses, Y. Becker, Editor. Martinus Nijhoff Publishing,Boston, pp. 73-86.
28. Honigman, A., H. Hyman and A.B. Oppenheim (1985). Analysis of viral transcription-termination signals, in: Viral Messenger RNA, Y. Becker,Editor. Martinus Nijhoff Publishing, Boston, pp. 61-75.
29. Honigman, A. and G. Nedjar-Pazerini (1986). Cloning and expression of the Lepidoptecan toxin produced by B. thuringiensis in E. coli. Gene. 42:69-77 
. 30. Hyman, H. and A. Honigman (1986). Transcription termination and processing sites in the lambda PL operon. J. Mol. Biol. 189:131-141. 31. Mador, N., A. Panet and A. Honigman (1989). Translation of gag pro and pol gene products of human T-cell leukemia virus type 2. J. Virology 63:2400-2404.
32. Unge, T., H. Ahola, K. Bhikhabhai, S. Lovgren, E. Fenyo, A. Honigman,A. Pane, J. Gronowirz and B. Strandberg (1990). Expression, purification and crystallization of the HIV-1 reverse transcriptase (RT). Aids Research and Human Retroviruses 6:1297-1303.
33. Honigman, A., D. Wolf, S. Yaish, H. Falk and A. Panet (1991).cis-acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus. Virol. 183:313-319.
34. Israel, S. and A. Honigman ( 1991). A bioluminescence assay for gene expression by continuous growing mammalian cells: application in detection of human immunodeficiency virus type I. Gene. 104:139-145.
35. Bar-Shira, A., A. Panet and A. Honigman (1991). An RNA secondary structure juxtaposes two remote genetic signals for HTLV-I RNA 3' end processing. J. Virol. 65:5165-5173.
36. Falk, H., N. Mador, R. Udi, A. Panet and A. Honigman (1993). Two cis-acting signals control ribosomal frameshift between human T-cell leukemia virus type II gag and pro genes. J. Virol. 67:6273-6277.
37. Kollmus, H., A. Honigman, A. Panet and H. Hauser (1994). Sequence and distance of slippery site and stem-loop define the strength of ribosomal frameshifting in HIV-1 and HTLV-2 in vivo. J. Virol. 68:6087-6091.
38. Honigman, A., H. Falk, N. Mador, T. Rosental and A. Panet (1995). Translation efficiency of the human T-cell leukemia virus (HTLV) gag gene modulates the frequency of ribosomal frameshifting. Virology 208:312-318.
39. Israel, S., M. Mendelovitz and A. Honigman(1995). Transactivation of human T-cell leukemia virus type 1 by Herpes simplex virus type 1. Virus Genes 9:269-276.
40. Nir-Paz, R., S. Israel, A. Honigman and I. Kahane (1995). Mycoplasmas regulate HIV-LTR-dependent gene expression. FEMS-Microbiol. Letters 128:63-68.
41. Wolf, D.G., Honigman, A., Lazarovits, J., Tavor, E. and A. Panet (1998). Characterization of the human cytomegalovirus UL97 gene product as a virion-associated protein kinase. Archives of Virology 143:1223-1232..
42. Wolf, D.G., Yaniv, I., Honigman, A., Kassis, I., Schonfeld, T., and S.Ashkenazi. (1998). Early emergence of ganciclovir-resistant human cytomegalovirus strains in children with primary combined immunodeficiency. J. Infect. Dis.178: 535-538
43. Honigman, A. Panet A. (1998) Structur and replication of HIV, In Aids, a global phenomenon. Editors: Morag, A and Honigman,A, Academon, The Hebrew University of Jerusalem, Student Union press., Jerusalem, Israel.pp.31-46
44. Axelrode J., Honigman, A., (1999) A sensitive and versatile bioluminescence bioassay for HIV-1 infection carried on Adenoviral vectors. AIDS research and human retroviruses.15: 759- 767.
45. Goren, I.,Tavor, E., and Honigman, A., (1999) Gene regulation mediated by interaction between HTLV-1 promoter elements and transcription factors TAX and CREB. Virology,258:303-312.
46. Segev, H, Honigman, A. Rosen, H. and Leitersdorf, A. (2000) Transcription regulation of sterol 27-hydroxylase gene and promoter mapping. Atheriosclerosis, accepted for publication.
Books
Aids A global Phenomenon (1998) Editors: Morag, A and Honigman, A, The Hebrew University of Jerusalem, Akademon, Student Union press.

Currant activities
Teaching:
During the last years I am deeply involved in teaching activities.
In addition to my regular teaching of under graduates and graduate students , as well as 4Graduate students doing their Ph.D. research in my laboratory, I served during the last 10 years on the curriculum committee of the Institute of Microbiology including chairing this committee for more then 4 years. The institute for Microbiology includes more the 50 professors and is teaching undergraduate and graduate students in all the aspects of microbiology and molecular biology.
In 1996 I founded an international teaching program which offers advanced courses in different subjects in Molecular Biology at the BioPharmaceutical Center Institute (BTCI) in Madison Wisconsin USA. In this program I am teaching an intensive course in Genetic Engineering "Current techniques in Protein and Genetic engineering". As a member of the curriculum committee of biotechnology of the Hebrew university for the last 10 years I am involved in defining the teaching program for the graduate students in this scientific area. As part of this program I was involved in a teaching exchange program with The GBF institute at Braunschweig Germany
Current Research
My research focuses on the molecular genetics of human retroviruses. As part of this study we designed a diagnostic bioassay for HIV based on bioluminescence. This highly sensitive and versatile assay can serve as a toll for determination of virus load in patients,as well as for automated drug screening.
Another topic that we study is the control of HTLV1 replication by the HTLV1 gene product Tax and it's effect on the cellular functions related to cancer and to oxygen stress.
In the last two years we started to develop a conditionally immortalized hepatocyte cell line that, we hope to apply, following transplantation, as a tool for bridging over the period until a suitable liver for transplantation is find.